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| Registros recuperados: 24 | |
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| Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed. |
| To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf |
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| Hussain, Mohamed Bakr; Laabir, Mohamed; Yahia, Mohamed Nejib Daly. |
| Copepods are excellent bioindicators of climate change and ecosystem pollution in anthropized coastal waters. This work reviewed the results of previous studies examining changes in egg production rate (EPR), hatching success (HS), and nauplius survival rate (NSR) in natural conditions and in the presence of pollutants, including heavy metals and organic contaminants such as polycyclic aromatic hydrocarbons (PAHs) and persistent organic pollutants (POPs). At high concentrations, cadmium and silver induce an increase in EPR in the copepods Acartia tonsa and Acartia hudsonica, while exposure to mercury decreases EPR in adults by 50%. All three metals affect HS, with mercury inducing a stronger effect than cadmium and silver. Cadmium affects reproductive... |
| Tipo: Text |
Palavras-chave: Planktonic copepods; Reproductive traits; Ecotoxicology. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00623/73498/72789.pdf |
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| Laabir, Mohamed; Grignon-dubois, Micheline; Masseret, Estelle; Rezzonico, Bernadette; Soteras, Giselle; Rouquette, Manuel; Rieuvilleneuve, Fabien; Cecchi, Philippe. |
| The inhibitory effects of crude extracts of Zostera marina L and Zostera noltii Hornemann on the growth of the toxic red tide dinoflagellate Alexandrium catenella were investigated through bio-assays. Methanolic and aqueous extracts were prepared from fresh and detrital leaves of Z. marina and Z. noltii collected in the Thau lagoon and Arcachon bay (France). All the extracts exhibited significant inhibition of A. catenella growth, whatever the species and without the need of continuous addition of extracts. The effective concentrations (EC50) varied in the range 0.036-0.199 g L-1 for Z. noltii and 0.036-0.239 g L-1 for Z. marina. Methanolic extracts prepared from fresh plant tissues were the most potent, with EC50 of 0.036-0.039 and 0.036-0.045 g L-1, for... |
| Tipo: Text |
Palavras-chave: Growth inhibition; Allelopathy; Alexandrium catenella; Zostera marina; Zostera noltii; Phenolics. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00170/28095/26720.pdf |
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| Jauzein, Cecile; Collos, Yves; Laabir, Mohamed; Vaquer, Andre. |
| Uptake rates of three potential N-sources (ammonium, nitrate and N-urea) and two potential C-sources (HCO(3)(-) and C-urea) were estimated during growth of Alexandrium catenella in both light and dark phases. According to the variations observed in (13)C-isotopic ratio, A. catenella cells were not able to use C-urea. Furthermore, decreases in (13)C cell content during darkness revealed a probably high involvement of C recently fixed in dark respiration. Dark N-uptake capacities of A. catenella were characterized by dark/light uptake ratio of 27%, 43% and 65% for NO(3)(-), NH(4)(+) and N-urea, respectively. An accumulation of C-rich compounds during the light period was highlighted through strong diel variations in C:N ratio and would provide C and energy... |
| Tipo: Text |
Palavras-chave: Diel cycle; Dark uptake; Nitrogen; Carbon; Uncoupling; Alexandrium catenella. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00056/16699/14199.pdf |
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| Abadie, Eric; Kaci, Lamia; Berteaux, Tom; Hess, Philipp; Sechet, Veronique; Masseret, Estelle; Rolland, Jean-luc; Laabir, Mohamed. |
| ulcanodinium rugosum, a recently described dinoflagellate species producing a potent neurotoxin (pinnatoxin G), has been identified in French Mediterranean lagoons and was responsible for recurrent episodes of shellfish toxicity detected by mouse bioassay. Until now, the biology and physiology of V. rugosum have not been fully investigated. We studied the growth characteristics and toxicity of a V. rugosum strain (IFR-VRU-01), isolated in the Ingril lagoon in June 2009 (North-Western French Mediterranean Sea). It was cultivated in Enriched Natural Sea Water (ENSW) with organic (urea) and inorganic (ammonium and nitrate) nitrogen, at a temperature of 25 °C and irradiance of 100 μmol/m2·s−1. Results showed that ammonium was assimilated by cells more rapidly... |
| Tipo: Text |
Palavras-chave: Vulcanodinium rugosum; Pinnatoxin G; Ingril lagoon; Growth; Nitrogen source. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00276/38748/37290.pdf |
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| Couet, Douglas; Pringault, Olivier; Bancon-montigny, Chrystelle; Briant, Nicolas; Poulichet, Francoise Elbaz; Delpoux, Sophie; Yahia, Ons Kefi-daly; Hela, Bengharbia; Charaf, M'Rabet; Herve, Fabienne; Rovillon, Georges-augustin; Amzil, Zouher; Laabir, Mohamed. |
| Controlled laboratory experiments were conducted to test the effects of copper (Cu2+) and butyltins (BuT) on the growth, photosynthetic activity and toxin content of two HABs (Harmful Algal Blooms) dinoflagellates, the planktonic Alexandrium catenella and the benthic Ostreopsis cf. ovata. Microalgae were exposed to increasing concentrations of Cu2+ (10−4 to 31 nM) or BuT (0.084 to 84 nM) for seven days. When considering the growth, EC50 values were 0.16 (±0.09) nM and 0.03 (±0.02) nM of Cu2+ for A. catenella and O. cf. ovata, respectively. Regarding BuT, EC50 was 14.2 (±6) nM for O. cf. ovata, while A. catenella growth inhibition appeared at BuT concentrations ≥27 nM. Photosynthetic activity of the studied dinoflagellates decreased with increasing Cu and... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella; Ostreopsis cf. ovata; Cu; Butyltin; Photosynthesis; Toxins. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00418/52970/53896.pdf |
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| Medhioub, Walid; Ramondenc, Simon; Vanhove, Audrey; Vergnes, Agnes; Masseret, Estelle; Savar, Veronique; Amzil, Zouher; Laabir, Mohamed; Rolland, Jean-luc. |
| This study assessed the apoptotic process occurring in the hemocytes of the Pacific oyster, Crassostrea gigas, exposed to Alexandrium catenella, a paralytic shellfish toxins (PSTs) producer. Oysters were experimentally exposed during 48 h to the toxic algae. PSTs accumulation, the expression of 12 key apoptotic-related genes, as well as the variation of the number of hemocytes in apoptosis was measured at time intervals during the experiment. Results show a significant increase of the number of hemocytes in apoptosis after 29 h of exposure. Two pro-apoptotic genes (Bax and Bax-like) implicated in the mitochondrial pathway were significantly upregulated at 21 h followed by the overexpression of two caspase executor genes (caspase-3 and caspase-7) at 29 h,... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxins; Apoptosis; Gene expression. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00176/28744/27213.pdf |
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| Abi-khalil, Celina; Lopez-joven, Carmen; Abadie, Eric; Savar, Veronique; Amzil, Zouher; Laabir, Mohamed; Rolland, Jean-luc. |
| The multifactorial etiology of massive Crassostrea gigas summer mortalities results from complex interactions between oysters, opportunistic pathogens and environmental factors. In a field survey conducted in 2014 in the Mediterranean Thau Lagoon (France), we evidenced that the development of the toxic dinoflagellate Alexandrium catenella, which produces paralytic shellfish toxins (PSTs), was concomitant with the accumulation of PSTs in oyster flesh and the occurrence of C. gigas mortalities. In order to investigate the possible role of toxic algae in this complex disease, we experimentally infected C. gigas oyster juveniles with Vibrio tasmaniensis strain LGP32, a strain associated with oyster summer mortalities, after oysters were exposed to Alexandrium... |
| Tipo: Text |
Palavras-chave: Harmful algae; Environment; Interaction; Pathogens; Defense; Paralytic shellfish toxin. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00309/42016/41308.pdf |
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| Triki, Habiba Zmerli; Laabir, Mohamed; Moeller, Peter; Chomerat, Nicolas; Daly-yahia, Ons Kefi. |
| The dinoflagellate Alexandrium pseudogonyaulax is widely distributed around the world including the Mediterranean waters. The objectives of this study were to determine the morphology and phylogenic affiliation of A. pseudogonyaulax strain isolated from Bizerte Lagoon (Mediterranean waters, Tunisia) and investigate its toxicity. Molecular analyses confirmed the morphological identification of the isolated strain (APBZ12) as A. pseudogonyaulax. Moreover, it showed that it is 100% identical with strains of this species found in New Zealand, Japan, China and North Sea (Norway and Denmark) suggesting that this species is cosmopolitan. Until now, no toxin studies have been conducted on fully characterized (morphologically and molecularly) A. pseudogonyaulax.... |
| Tipo: Text |
Palavras-chave: Alexandrium pseudogonyaulax; Goniodomin A; Mediterranean sea; Morphology; Phylogeny. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00302/41356/40548.pdf |
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| Gueguen, Marielle; Lassus, Patrick; Laabir, Mohamed; Bardouil, Michele; Baron, Regis; Sechet, Veronique; Truquet, Philippe; Amzil, Zouher; Barille, Laurent. |
| The occurrence of new phytoplankton species in a coastal area may be explained by the import of shellfish containing whole live algal cells in their digestive tracts. Indeed, shellfish containing toxic algal cells can induce both primary contaminations in safe areas ( initially free from toxic microalgae), and secondary contaminations of other shellfish growing in the same area via the faeces of the imported animals. To mitigate this problem, shellfish need to be placed in a separate holding tank and their intestinal content purged. For a deeper understanding of the risks associated with transferring contaminated shellfish, oysters ( Crassostrea gigas) and mussels ( Mytilus edulis) were purposely fed either Alexandrium minutum or A. catenella (... |
| Tipo: Text |
Palavras-chave: Risk assessment; Toxic phytoplankton; Uptake; Gut passage; Mytilus edulis; Crassostrea gigas. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3897.pdf |
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| Laabir, Mohamed; Collos, Yves; Masseret, Estelle; Grzebyk, Daniel; Abadie, Eric; Savar, Veronique; Sibat, Manoella; Amzil, Zouher. |
| Laboratory experiments were designed to study the toxin content and profile of the Alexandrium catenella strain ACT03 (isolated from Thau Lagoon, French Mediterranean) in response to abiotic environmental factors under nutrient-replete conditions. This dinoflagellate can produce various paralytic shellfish toxins with concentrations ranging from 2.9 to 50.3 fmol/cell. The toxin profile was characterized by carbamate toxins (GTX3, GTX4 and GTX5) and N-sulfocarbamoyl toxins (C1, C2, C3 and C4). C2 dominated at 12-18 degrees C, but only for salinities ranging from 10 to 25 psu, whereas GTX5 became dominant at temperatures ranging from 21 to 30 degrees C at almost all salinities. There was no significant variation in the cellular toxin amount from 18 degrees C... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella; PSP toxins; Temperature; Salinity; Light. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25969/24056.pdf |
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| Chetouhi, Cherif; Masseret, Estelle; Satta, Cecilia Teodora; Balliau, Thierry; Laabir, Mohamed; Jean, Natacha. |
| Over the past decades, the occurrence, distribution and intensity of harmful algal blooms involving the dinoflagellate Alexandrium pacificum have increased in marine coastal areas disturbed by anthropogenic inputs. This invasive species produces saxitoxin, which causes the paralytic shellfish poisoning syndrome in humans upon consumption of contaminated seafood. Blooms of A. pacificum have been reported in metal-contaminated coastal ecosystems, suggesting some ability of these microorganisms to adapt to and/or resist in metal stress conditions. This study seeks to characterize the modifications in membrane proteomes (by 2-D electrophoresis coupled to LC-MS/MS), cell growth and morphometry (measured with an inverted microscope), in response to metal stress... |
| Tipo: Text |
Palavras-chave: Alexandrium pacificum; Harmful algal bloom; Proteomics; Membrane pmteome; Trace metals. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71802/70290.pdf |
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| Laporte, Martin; Shao, Zhaojun; Berrebi, Patrick; Laabir, Mohamed; Abadie, Eric; Faivre, Nicolas; Rieuvilleneuve, Fabien; Masseret, Estelle. |
| Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic... |
| Tipo: Text |
Palavras-chave: Invasive species; Phytoplankton; Alexandrium; Dinophyceae; Microsatellite markers; Biogeography. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00189/30026/29398.pdf |
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| Lassus, Patrick; Amzil, Zouher; Bardouil, Michele; Baron, Regis; Bohec, Madeleine; Bougrier, Serge; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Sechet, Veronique; Bablon, Clotilde; Berard, Jean-baptiste; Carre, Camille; Cheignon, Valerie; Lecamp, Sylvain; Mabit, Marie. |
| L'objectif général de l'étude est de simuler les épisodes de contamination de certains bivalves (huîtres et moules) par les toxines d'Alexandrium minutum et A. calenella à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à ces espèces. Ce modèle sera ensuite susceptible d'être couplé aux modèles biologiques locaux (estuaire de Penzé et étang de Thau) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer, Université de Montpellier). La première année (2001-2002) était consacrée à la simulation des contaminations en estuaire de Penzé (Alexandrium minulum) et au développement de cultures en masse d'A. calenella de Thau en bioréacteurs. |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00156/26711/24786.pdf |
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| Lassus, Patrick; Bardouil, Michele; Baron, Regis; Garen, Pierre; Laabir, Mohamed; Leguay, Didier; Masselin, Pierre; Truquet, Philippe; Joret, Camille. |
| L'objectif de l'étude est de simuler les épisodes de contamination des huîtres par les toxines d'Alexandrium minufum à partir d'un module expérimental adapté, et en vue de construire un modèle de contamination applicable à cette espèce. Ce modèle sera ensuite susceptible d'être couplé au modèle biologique local (estuaire de Penzé) de déterminisme des blooms, ainsi qu'aux modèles hydrodynamiques développés par différentes équipes (Ifremer). |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00156/26712/24787.pdf |
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| Ben Gharbia, Hela; Yahia, Ons Kefi-daly; Cecchi, Philippe; Masseret, Estelle; Amzil, Zouher; Herve, Fabienne; Rovillon, Georges-augustin; Nouri, Habiba; M'Rabet, Charaf; Couet, Douglas; Triki, Habiba Zmerli; Laabir, Mohamed. |
| Macrophytes are known to release allelochemicals that have the ability to inhibit the proliferation of their competitors. Here, we investigated the effects of the fresh leaves of two magnoliophytes (Zostera noltei and Cymodocea nodosa) and thalli of the macroalgae Ulva rigida on three HAB-forming benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima, and Coolia monotis). The effects of C. nodosa and U. rigida were also tested against the neurotoxic planktonic dinoflagellate Alexandrium pacificum Litaker sp. nov (former Alexandrium catenella). Co-culture experiments were conducted under controlled laboratory conditions and potential allelopathic effects of the macrophytes on the growth, photosynthesis and toxin production of the targeted... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00411/52200/52917.pdf |
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| Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine. |
| Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French... |
| Tipo: Text |
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf |
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| Collos, Yves; Hadjadji, Imene; Plisson, Benoit; Cecchi, Philippe; Laabir, Mohamed; Bechemin, Christian; Masseret, Estelle. |
| Alexandrium tamarense (M. Lebour) Balech strains isolated in spring 2007 from a single bloom in Thau lagoon have been grown in nonaxenic artificial media. For three strains showing large oscillations in biomass (crashes followed by recoveries) on a scale of several days, a significant relationship was observed between changes in cell densities (as in vivo fluorescence) and changes in nitrate concentrations. Increases in cell densities were accompanied by decreases in nitrate, while decreases in cell densities corresponded to increases in nitrate, presumably due to nitrification. Net increases in nitrate could reach up to 15 mu mol N . L(-1) . d(-1) indicating a very active nitrifying archaeal/bacterial population. However, following population crashes,... |
| Tipo: Text |
Palavras-chave: Alexandrium tamarense; Archaea; Bacteria; Decay; Growth; Nitrate; Nitrification; Oscillations. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00049/16046/13585.pdf |
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| Ben-gharbia, Hela; Yahia, Ons Kefi-daly; Amzil, Zouher; Chomerat, Nicolas; Abadie, Eric; Masseret, Estelle; Sibat, Manoella; Triki, Habiba Zmerli; Nouri, Habiba; Laabir, Mohamed. |
| armful benthic dinoflagellates, usually developing in tropical areas, are expanding to temperate ecosystems facing water warming. Reports on harmful benthic species are particularly scarce in the Southern Mediterranean Sea. For the first time, three thermophilic benthic dinoflagellates (Ostreopsis cf. ovata, Prorocentrum lima and Coolia monotis) were isolated from Bizerte Bay (Tunisia, Mediterranean) and monoclonal cultures established. The ribotyping confirmed the morphological identification of the three species. Maximum growth rates were 0.59 ± 0.08 d−1 for O. cf. ovata, 0.35 ± 0.01 d−1 for C. monotis and 0.33 ± 0.04 d−1 for P. lima. Toxin analyses revealed the presence of ovatoxin-a and ovatoxin-b in O. cf. ovata cells. Okadaic acid and... |
| Tipo: Text |
Palavras-chave: Ostreopsis cf. ovata; Prorocentrum lima; Coolia monotis; Southern Mediterranean Sea; Growth; Toxicity. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00355/46585/46393.pdf |
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| Registros recuperados: 24 | |
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